SubtiBank SubtiBank
ytnA [2019-10-22 13:14:48]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ytnA [2019-10-22 13:14:48]

similar to proline permease
locus
BSU_30530
pI
9.91
mw
50.16 kDa
protein length
463 aa Sequence Blast
gene length
1392 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.4|APC superfamily]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.15|Biosynthesis/acquisition of L- and D-alanine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,124,250 3,125,641

    The protein

    Protein family

  • [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|423AEC9D260BE53AD22851D999244B89BEB3E84D|HutM], [protein|8C4A8961B2514A8AD531AFB104B10C0797D02AC2|YvbW], [protein|9AD0E43119BDC65D5F67CE55CD9417E1E6BAE058|YbxG], [protein|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|RocC], [protein|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|RocE], [protein|B6A920650919D5BBBD75CCA58C30A0D8ECE64DA3|YbgF], [protein|C7FEA1D9C7C96C39CEE83CE3F13D412DE6AFE10E|YdgF], [protein|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|AapA], [protein|1594BA83CED2DA9F86C1CE2A0DC6E1C9B665EACB|GabP]

    • Structure

  • [PDB|6F34] (from Geobacillus kaustophilus, 21% identity) [pubmed|29416041]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB]-[gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]
    description
    [Pubmed|10498721]

    regulation

  • constitutive
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A126 (ytnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/126 NBRP B. subtilis, Japan]
  • GP1885 (Δ[gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]::''spc'') available in [SW|Jörg Stülke]'s lab
  • BKE30530 ([gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT
  • BKK30530 ([gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT

    • Expression vector

  • pGP2280: expression of ''ytnA'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP2276 (in [SW|pAC5]) (GP2963), available in [SW|Jörg Stülke]'s lab

    • References

  • 10498721,29416041